LOCUS       40924_9126        5003 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Plant expression vector pCB302 LIC, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5003)
  AUTHORS   Zurcher E, Liu J, di Donato M, Geisler M, Muller B.
  TITLE     Plant development regulated by cytokinin sinks
  JOURNAL   Science 353 (6303), 1027-1030 (2016)
  PUBMED    27701112
REFERENCE   2  (bases 1 to 5003)
  AUTHORS   Muller B.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUL-2016) Plant and Microbial Biology, University of 
            Zurich, Zollikerstrasse 107, Zurich, ZH 8008, Switzerland
REFERENCE   3  (bases 1 to 5003)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5003)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science"; 
            date: "2016"; volume: "353"; issue: "6303"; pages: "1027-1030"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-JUL-2016) Plant and Microbial Biology, University of Zurich, 
            Zollikerstrasse 107, Zurich, ZH 8008, Switzerland"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5003
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(225..249)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        406..589
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             589..1137
                     /codon_start=1
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      1161..1413
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_recomb     1440..1470
                     /label=LIC, ligation-independent cloning (LIC) site
                     /note="LIC, ligation-independent cloning (LIC) site"
     misc_feature    1541..1565
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(1720..2865)
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(3167..3958)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      complement(4170..4801)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    4879..4903
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     4984..5000
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 agctttagcc gacccagacg agccaaggga tctttttgga atgctgctcc gtcgtcaggc
       61 tttccgacgt ttgggtggtt gaacagaagt cattatcgta cggaatgcca agcactcccg
      121 aggggaaccc tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc
      181 ccttttaaat atccgttatt ctaataaacg ctcttttctc ttaggtttac ccgccaatat
      241 atcctgtcaa acactgatag tttaaactga aggcgggaaa cgacaatctg atcatgagcg
      301 gagaattaag ggagtcacgt tatgaccccc gccgatgacg cgggacaagc cgttttacgt
      361 ttggaactga cagaaccgca acgattgaag gagccactca gccgcgggtt tctggagttt
      421 aatgagctaa gcacatacgt cagaaaccat tattgcgcgt tcaaaagtcg cctaaggtca
      481 ctatcagcta gcaaatattt cttgtcaaaa atgctccact gacgttccat aaattcccct
      541 cggtatccaa ttagagtctc atattcactc tcaatccaaa taatctgcat gagcccagaa
      601 cgacgcccgg ccgacatccg ccgtgccacc gaggcggaca tgccggcggt ctgcaccatc
      661 gtcaaccact acatcgagac aagcacggtc aacttccgta ccgagccgca ggaaccgcag
      721 gagtggacgg acgacctcgt ccgtctgcgg gagcgctatc cctggctcgt cgccgaggtg
      781 gacggcgagg tcgccggcat cgcctacgcg ggcccctgga aggcacgcaa cgcctacgac
      841 tggacggccg agtcgaccgt gtacgtctcc ccccgccacc agcggacggg actgggctcc
      901 acgctctaca cccacctgct gaagtccctg gaggcacagg gcttcaagag cgtggtcgct
      961 gtcatcgggc tgcccaacga cccgagcgtg cgcatgcacg aggcgctcgg atatgccccc
     1021 cgcggcatgc tgcgggcggc cggcttcaag cacgggaact ggcatgacgt gggtttctgg
     1081 cagctggact tcagcctgcc ggtaccgccc cgtccggtcc tgcccgtcac cgagatctaa
     1141 ggatcagctc gaatttcccc gatcgttcaa acatttggca ataaagtttc ttaagattga
     1201 atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg
     1261 taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc
     1321 cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat
     1381 tatcgcgcgc ggtgtcatct atgttactag atcgggaatt cctgcagccc gggggatcct
     1441 agttggaatg ggttaaccca actccataag tctagagcgg ccgccaccgc ggtggagctc
     1501 agattgtcgt ttcccgcctt cagtttaaac tatcagtgtt tgacaggata tattggcggg
     1561 taaacctaag agaaaagagc gtttattaga ataatcggat atttaaaagg gcgtgaaaag
     1621 gtttatccgt tcgtccattt gtatgtgcat gccaaccaca ggagatctca gtaaagcgct
     1681 ggctgaaccc ccagccggaa ctgaccccac aaggccctag cgtttgcaat gcaccaggtc
     1741 atcattgacc caggcgtgtt ccaccaggcc gctgcctcgc aactcttcgc aggcttcgcc
     1801 gacctgctcg cgccacttct tcacgcgggt ggaatccgat ccgcacatga ggcggaaggt
     1861 ttccagcttg agcgggtacg gctcccggtg cgagctgaaa tagtcgaaca tccgtcgggc
     1921 cgtcggcgac agcttgcggt acttctccca tatgaatttc gtgtagtggt cgccagcaaa
     1981 cagcacgacg atttcctcgt cgatcaggac ctggcaacgg gacgttttct tgccacggtc
     2041 caggacgcgg aagcggtgca gcagcgacac cgattccagg tgcccaacgc ggtcggacgt
     2101 gaagcccatc gccgtcgcct gtaggcgcga caggcattcc tcggccttcg tgtaataccg
     2161 gccattgatc gaccagccca ggtcctggca aagctcgtag aacgtgaagg tgatcggctc
     2221 gccgataggg gtgcgcttcg cgtactccaa cacctgctgc cacaccagtt cgtcatcgtc
     2281 ggcccgcagc tcgacgccgg tgtaggtgat cttcacgtcc ttgttgacgt ggaaaatgac
     2341 cttgttttgc agcgcctcgc gcgggatttt cttgttgcgc gtggtgaaca gggcagagcg
     2401 ggccgtgtcg tttggcatcg ctcgcatcgt gtccggccac ggcgcaatat cgaacaagga
     2461 aagctgcatt tccttgatct gctgcttcgt gtgtttcagc aacgcggcct gcttggcctc
     2521 gctgacctgt tttgccaggt cctcgccggc ggtttttcgc ttcttggtcg tcatagttcc
     2581 tcgcgtgtcg atggtcatcg acttcgccaa acctgccgcc tcctgttcga gacgacgcga
     2641 acgctccacg gcggccgatg gcgcgggcag ggcaggggga gccagttgca cgctgtcgcg
     2701 ctcgatcttg gccgtagctt gctggaccat cgagccgacg gactggaagg tttcgcgggg
     2761 cgcacgcatg acggtgcggc ttgcgatggt ttcggcatcc tcggcggaaa accccgcgtc
     2821 gatcagttct tgcctgtatg ccttccggtc aaacgtccga ttcattcacc ctccttgcgg
     2881 gattgccccg actcacgccg gggcaatgtg cccttattcc tgatttgacc cgcctggtgc
     2941 cttggtgtcc agataatcca ccttatcggc aatgaagtcg gtcccgtaga ccgtctggcc
     3001 gtccttctcg tacttggtat tccgaatctt gccctgcacg aataccagcg accccttgcc
     3061 caaatacttg ccgtgggcct cggcctgaga gccaaaacac ttgatgcgga agaagtcggt
     3121 gcgctcctgc ttgtcgccgg catcgttgcg ccacatctag gtactaaaac aattcatcca
     3181 gtaaaatata atattttatt ttctcccaat caggcttgat ccccagtaag tcaaaaaata
     3241 gctcgacata ctgttcttcc ccgatatcct ccctgatcga ccggacgcag aaggcaatgt
     3301 cataccactt gtccgccctg ccgcttctcc caagatcaat aaagccactt actttgccat
     3361 ctttcacaaa gatgttgctg tctcccaggt cgccgtggga aaagacaagt tcctcttcgg
     3421 gcttttccgt ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga gtgtcttctt
     3481 cccagttttc gcaatccaca tcggccagat cgttattcag taagtaatcc aattcggcta
     3541 agcggctgtc taagctattc gtatagggac aatccgatat gtcgatggag tgaaagagcc
     3601 tgatgcactc cgcatacagc tcgataatct tttcagggct ttgttcatct tcatactctt
     3661 ccgagcaaag gacgccatcg gcctcactca tgagcagatt gctccagcca tcatgccgtt
     3721 caaagtgcag gacctttgga acaggcagct ttccttccag ccatagcatc atgtcctttt
     3781 cccgttccac atcataggtg gtccctttat accggctgtc cgtcattttt aaatataggt
     3841 tttcattttc tcccaccagc ttatatacct tagcaggaga cattccttcc gtatctttta
     3901 cgcagcggta tttttcgatc agttttttca attccggtga tattctcatt ttagccattt
     3961 attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta attataacaa
     4021 gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga aaacagcttt
     4081 ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga ttttgaaacc
     4141 acaattatgg gtgatgctgc caactcgaga gcgggccggg agggttcgag aagggggggc
     4201 accccccttc ggcgtgcgcg gtcacgcgca cagggcgcag ccctggttaa aaacaaggtt
     4261 tataaatatt ggtttaaaag caggttaaaa gacaggttag cggtggccga aaaacgggcg
     4321 gaaacccttg caaatgctgg attttctgcc tgtggacagc ccctcaaatg tcaataggtg
     4381 cgcccctcat ctgtcagcac tctgcccctc aagtgtcaag gatcgcgccc ctcatctgtc
     4441 agtagtcgcg cccctcaagt gtcaataccg cagggcactt atccccaggc ttgtccacat
     4501 catctgtggg aaactcgcgt aaaatcaggc gttttcgccg atttgcgagg ctggccagct
     4561 ccacgtcgcc ggccgaaatc gagcctgccc ctcatctgtc aacgccgcgc cgggtgagtc
     4621 ggcccctcaa gtgtcaacgt ccgcccctca tctgtcagtg agggccaagt tttccgcgag
     4681 gtatccacaa cgccggcggc cgcggtgtct cgcacacggc ttcgacggcg tttctggcgc
     4741 gtttgcaggg ccatagacgg ccgccagccc agcggcgagg gcaaccagcc cggtgagcgt
     4801 ctagtggact gatgggctgc ctgtatcgag tggtgatttt gtgccgagct gccggtcggg
     4861 gagctgttgg ctggctggtg gcaggatata ttgtggtgta aacaaattga cgcttagaca
     4921 acttaataac acattgcgga cgtttttaat gtactggggt ggttttggta ccgggccccc
     4981 cctcgaggtc gacggtatcg ata
//
